A Quantitative, Facile, and High-Throughput Image-Based Cell Migration Method Is a Robust Alternative to the Scratch Assay
نویسندگان
چکیده
منابع مشابه
A quantitative, facile, and high-throughput image-based cell migration method is a robust alternative to the scratch assay.
Cell migration is a key phenotype for a number of therapeutically important biological responses, including angiogenesis. A commonly used method to assess cell migration is the scratch assay, which measures the movement of cells into a wound made by physically scoring a confluent cell monolayer to create an area devoid of cells. Although this method has been adequate for qualitative characteriz...
متن کاملA high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods
BACKGROUND Cell migration is a complex phenomenon that requires the coordination of numerous cellular processes. Investigation of cell migration and its underlying biology is of interest to basic scientists and those in search of therapeutics. Current migration assays for screening small molecules, siRNAs, or other perturbations are difficult to perform in parallel at the scale required to scre...
متن کاملon the practicality and effectiveness of a personalized eclectic method incorporated into iranian high school efl syllabus
همگام با سرعت در حال رشد خلاقیت و نوآوری های آموزش زبان به ویژه ظهور روش ارتباطی آموزش زبان? بسیاری از مدارس زبان با بازاندیشی آموزش و پرورش خود? برای گنجاندن فعالیت های ارتباطی، وزمینه ی شخصی سازی شده به شیوه های سنتی خود به روز رسانی شده اند. با این حال، مدارس ایرانی در این زمینه آهسته پیش رفته اند. از این رو، هدف عمده ی پژوهش حاضر برداشتن یک گام در پر کردن شکاف بین نظریه های آموزشی نو ظهور و...
15 صفحه اولA high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis
There is a growing demand for in vitro assays for toxicity screening in three-dimensional (3D) environments. In this study, 3D cell culture using magnetic levitation was used to create an assay in which cells were patterned into 3D rings that close over time. The rate of closure was determined from time-lapse images taken with a mobile device and related to drug concentration. Rings of human em...
متن کاملDesign of a high-throughput human neural crest cell migration assay to indicate potential developmental toxicants.
Migration of neural crest cells (NCCs) is one of the pivotal processes of human fetal development. Malformations arise if NCC migration and differentiation are impaired genetically or by toxicants. In the currently available test systems for migration inhibition of NCC (MINC), the manual generation of a cell-free space results in extreme operator dependencies, and limits throughput. Here a new ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biomolecular Screening
سال: 2011
ISSN: 1087-0571,1552-454X
DOI: 10.1177/1087057110393340